The proposed Indiana University School of Medicine (IUSM) Cooperative Center of Excellence in Hematology (CCEH) brings together 23 investigators whose research activities are focused on various aspects of nonmalignant hematology and whose work is highly dependent on one or more of the four biomedical research cores supported by the Center. The central theme of our CCEH is the regulation of human and murine hematopoiesis at the level of hematopoietic stem (HSC) and progenitor (HPC) cells. The goal of the investigations of members of this center is to leverage different components of the hematopoietic system to improve the advancement of the clinical utility and efficacy of HSC/HPC-based therapies. We believe that in order to attain these goals, we must understand basic biological processes that affect hematopoietic stem cell behavior both in vitro and in vivo in a basic science laboratory and to eventually establish clinical trials that transform these findings into translational efforts. The CCEH membership draws from a group of well-funded investigators with a diverse but complementary experience in experimental and clinical stem cell transplantation, signaling in and regulation of HSC and HPC, developmental emergence of fetal hematopoiesis, gene transfer, interactions between HSC and the hematopoietic niche, mobilization, homing and engraftment of HSC, modulation of function of freshly isolated and ex vivo manipulated HSC, and functional status of HSC after exposure to ionizing radiation. The cores of the CCEH provide essential and in some case, unique, services to CCEH members. These are: Experimental Mouse Resources, Optical Microscopy, Angiogenesis, and Flow Cytometry cores. All cores will support the basic and translational studies that underlie the mission of the CCEH and facilitate development of new discoveries into human trials. The CCEH also includes a Pilot and Feasibility Project to enhance the training of young investigators and advance their careers in non-malignant hematology. The Center also sponsors a well-developed enrichment program to advance the development of both young and established CCEH members.